Abstract
Serological methods such as the enzyme-linked immunosorbent assay (ELISA) and virus neutralization test are fundamental tools used in diagnosis, seroepidemiological studies of filovirus transmission/prevalence, and the evaluation of vaccine immunogenicity and potential therapeutic antibodies. Filoviruses have a single transmembrane glycoprotein (GP), which is the only known target of neutralizing antibodies. Here we describe serological methods to quantify filovirus GP-specific antibodies.
Keywords:
ADE; Antibody; Antibody-dependent enhancement; ELISA; Ebolavirus; Filovirus; Glycoprotein; Marburgvirus; Neutralization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Neutralizing / immunology
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Antibodies, Neutralizing / isolation & purification*
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Antibodies, Viral / immunology
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Antibodies, Viral / isolation & purification
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Ebolavirus / immunology
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Ebolavirus / pathogenicity
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Enzyme-Linked Immunosorbent Assay / methods*
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Glycoproteins / immunology
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Glycoproteins / isolation & purification
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Hemorrhagic Fever, Ebola / immunology*
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Hemorrhagic Fever, Ebola / prevention & control
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Hemorrhagic Fever, Ebola / virology
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Humans
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Viral Vaccines / immunology*
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Viral Vaccines / pharmacology
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Glycoproteins
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Viral Vaccines