A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay

J Microbiol Methods. 2017 Aug:139:165-167. doi: 10.1016/j.mimet.2017.06.001. Epub 2017 Jun 2.

Abstract

The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.

Keywords: 16s rDNA; Fluorescence Resonance Energy Transfer; Gold nanoparticles; In vitro diagnosis; Mycobacterium tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal / isolation & purification*
  • Fluorescence Resonance Energy Transfer / economics
  • Fluorescence Resonance Energy Transfer / methods*
  • Gold
  • Humans
  • Metal Nanoparticles*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification*
  • RNA, Ribosomal, 16S / genetics
  • Sensitivity and Specificity
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Gold