Serum Levels of MicroRNA371a-3p: A Highly Sensitive Tool for Diagnosing and Staging Testicular Germ Cell Tumours: A Clinical Case Series

Urol Int. 2017;99(1):98-103. doi: 10.1159/000477446. Epub 2017 Jun 7.

Abstract

Introduction: MicroRNA (miR)371a-3p was suggested to be a sensitive and specific new serum biomarker of germ cell tumours (GCTs); however, its clinical usefulness remains unproven.

Patients, methods: In 312 consecutive cases with various testicular diseases, serum levels of miR371a-3p were measured. Measurement results became available only after completion of treatment. Five patients with testicular seminoma were selected for review because of unanticipated clinical courses.

Results: In each two patients, elevated miR levels heralded undetected primary testicular GCT and metastases despite inconclusive radiological findings. In one case, a normal miR371a-3p level correctly pointed to the absence of metastases contrary to clinical assessment. In all cases, knowledge about the miR371a-3p levels would have altered the clinical management.

Conclusions: These cases highlight the exceptional usefulness of the new GCT biomarker. In contrast to classical markers, miR371a-3p can identify primary testicular GCT. The marker can aid in clinical decision making in cases with ambiguous clinical findings.

Keywords: Biomarker; MicroRNA371a-3p; Testicular germ cell tumour; Treatment monitoring.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics*
  • Circulating MicroRNA / blood
  • Circulating MicroRNA / genetics*
  • Clinical Decision-Making
  • Humans
  • Male
  • MicroRNAs / blood
  • MicroRNAs / genetics*
  • Neoplasm Staging / methods*
  • Neoplasms, Germ Cell and Embryonal / blood
  • Neoplasms, Germ Cell and Embryonal / genetics*
  • Neoplasms, Germ Cell and Embryonal / secondary
  • Neoplasms, Germ Cell and Embryonal / therapy
  • Polymerase Chain Reaction*
  • Predictive Value of Tests
  • Seminoma / blood
  • Seminoma / genetics*
  • Seminoma / secondary
  • Seminoma / therapy
  • Testicular Neoplasms / blood
  • Testicular Neoplasms / genetics*
  • Testicular Neoplasms / pathology
  • Testicular Neoplasms / therapy
  • Tomography, X-Ray Computed
  • Ultrasonography
  • Young Adult

Substances

  • Biomarkers, Tumor
  • Circulating MicroRNA
  • MIRN371 microRNA, human
  • MicroRNAs

Supplementary concepts

  • Testicular Germ Cell Tumor