Co-expression of glucose-6-phosphate dehydrogenase and acyl-CoA binding protein enhances lipid accumulation in the yeast Yarrowia lipolytica

N Biotechnol. 2017 Oct 25;39(Pt A):18-21. doi: 10.1016/j.nbt.2017.05.008. Epub 2017 Jun 4.

Abstract

The oleaginous yeast Yarrowia lipolytica is a convenient model for investigating lipid biosynthesis and for engineering high lipid accumulated strains. In this organism, the pentose phosphate pathway is the major source of NADPH for lipid biosynthesis. Thus, we over-expressed gene encoding NADP+-dependent glucose-6-phosphate dehydrogenase (ZWF1) in a strain deficient in peroxisome biogenesis. However, this strategy suppressed growth during cultivation under lipogenic conditions and did not significantly increase lipid accumulation. Remarkably, co-expression of gene encoding acyl-CoA binding protein (ACBP), which functions as an intracellular acyl-CoA transporter and acyl-CoA-pool former, restored growth. Co-expression of ZWF1 and ACBP increased the lipid content to 30% of dry cell weight via de novo lipid synthesis. In comparison to wild type, the engineered strain accumulated 41% more lipids with a higher ratio of saturated to unsaturated fatty acids.

Keywords: Acyl-CoA binding protein; Lipid accumulation; NADP(+)-dependent glucose-6-phosphate dehydrogenase; Yarrowia lipolytica.

MeSH terms

  • Diazepam Binding Inhibitor / genetics
  • Diazepam Binding Inhibitor / metabolism*
  • Gene Expression*
  • Genes, Fungal
  • Glucosephosphate Dehydrogenase / genetics
  • Glucosephosphate Dehydrogenase / metabolism*
  • Lipid Metabolism*
  • Plasmids / metabolism
  • Yarrowia / genetics
  • Yarrowia / metabolism*

Substances

  • Diazepam Binding Inhibitor
  • Glucosephosphate Dehydrogenase