Elevated sodium leads to the increased expression of HSP60 and induces apoptosis in HUVECs

PLoS One. 2017 Jun 12;12(6):e0179383. doi: 10.1371/journal.pone.0179383. eCollection 2017.

Abstract

Atherosclerosis is the leading cause of death in the world. We have previously shown that expression of heat shock protein 60 (HSP60) on the surface of endothelial cells is the main cause of initiating the disease as it acts as a T cell auto-antigen and can be triggered by classical atherosclerosis risk factors, such as infection (e.g. Chlamydia pneumoniae), chemical stress (smoking, oxygen radicals, drugs), physical insult (heat, shear blood flow) and inflammation (inflammatory cytokines, lipopolysaccharide, oxidized low density lipoprotein, advanced glycation end products). In the present study, we show that increasing levels of sodium chloride can also induce an increase in intracellular and surface expression of HSP60 protein in human umbilical vein endothelial cells. In addition, we found that elevated sodium induces apoptosis.

MeSH terms

  • Apoptosis* / drug effects
  • Apoptosis* / genetics
  • Biomarkers
  • Chaperonin 60 / genetics*
  • Chaperonin 60 / metabolism
  • Gene Expression Regulation* / drug effects
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Human Umbilical Vein Endothelial Cells / metabolism*
  • Humans
  • Immunophenotyping
  • Protein Transport
  • Sodium / metabolism*
  • Sodium / pharmacology

Substances

  • Biomarkers
  • Chaperonin 60
  • Sodium

Grants and funding

B.J. received a grant from the Tiroler Wissenschaftfonds, grant no: UNI‐0404/1395. Website: https://www.tirol.gv.at/bildung/wissenschaftsfonds/. B.J. received a donation from the Lore and Udo Saldow donation. G.W. received a grant from Österreischische Nationalbank, grant no 15953. Website: https://www.oenb.at/Ueber-Uns/Forschungsfoerderung/Jubilaeumsfonds.html. The funders and donors had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.