Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis

Yuan-Feng Zou; Yu-Ping Fu; Xing-Fu Chen; Ingvild Austarheim; Kari Tvete Inngjerdingen; Chao Huang; Fei-Yi Lei; Xu Song; Lixia Li; Gang Ye; Lemlem Dugassa Eticha; Zhongqiong Yin; Berit Smestad Paulsen

doi:10.1016/j.carbpol.2017.04.049

Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis

Carbohydr Polym. 2017 Sep 15:172:306-314. doi: 10.1016/j.carbpol.2017.04.049. Epub 2017 Apr 23.

Authors

Affiliations

¹ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, 611130 Wenjiang, PR China.
² Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, 611130 Wenjiang, PR China. Electronic address: [email protected].
³ Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
⁴ Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, 611130 Wenjiang, PR China.

PMID: 28606539
DOI: 10.1016/j.carbpol.2017.04.049

Abstract

Two polysaccharides, GCP-I-I and GCP-II-I, were obtained from 100°C water extracts of Gentiana crassicaulis roots by DEAE anion exchange chromatography and gel filtration. The results from methanolysis, methylation, FT-IR and NMR, indicated that these two fractions are typical pectic polysaccharides, with HG and RG-I regions and AG-I/AG-II side chains, and some of the galacturonic acid units of fraction GCP-I-I were methyl esterified. Fractions GCP-I-I and GCP-II-I, both exhibited potent complement fixation, and fraction GCP-I-I was more potent than positive control BPII. The higher complement fixation activity obtained in fraction GCP-I-I may be due to the higher Mw and/or higher amount of AG-II present in fraction GCP-I-I than fraction GCP-II-I. The polysaccharides from G. crassicaulis could be used as a potential natural immunomodulator.

Keywords: Complement system; Gentiana crassicaulis; Polysaccharides; Purification; Trifluoroacetic acid (PubChem CID: 6422); d-Fructose (PubChem CID: 5984); d-Galactose (PubChem CID: 6036); d-Galacturonic acid (PubChem CID: 439215); d-Glucose (PubChem CID: 5793); d-Glucuronic acid (PubChem CID: 94715); d-Mannose (PubChem CID: 18950); d-Xylose (PubChem CID: 135191); l-Arabinose (PubChem CID: 439195); l-Rhamnose (PubChem CID: 25310).

MeSH terms

Animals
Chromatography, Gel
Complement Fixation Tests
Erythrocytes / drug effects*
Gentiana / chemistry*
Plant Roots / chemistry*
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*
Sheep
Spectroscopy, Fourier Transform Infrared

Substances

Polysaccharides