Characterization of Novel Collagenolytic Proteases

Goran Mucić; Brankica Rašković; Natalija Polović

doi:10.1007/978-1-4939-7111-4_7

Characterization of Novel Collagenolytic Proteases

Methods Mol Biol. 2017:1626:71-78. doi: 10.1007/978-1-4939-7111-4_7.

Authors

Goran Mucić¹, Brankica Rašković², Natalija Polović²

Affiliations

¹ Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia. [email protected].
² Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.

PMID: 28608201
DOI: 10.1007/978-1-4939-7111-4_7

Abstract

Collagenolytic proteases have many potential applications in different areas of science, industry, and medicine. The determination of the activity of such proteins is paramount to their application. Here, we describe methods which can be applied to determine the activity and some basic characteristics of potential collagenases.

Keywords: BAPNA assay; Caseinolytic activity; Collagen; Collagenase; Collagenolytic activity.

MeSH terms

Animals
Collagen / metabolism
Collagenases / analysis
Collagenases / metabolism*
Electrophoresis, Polyacrylamide Gel / methods*
Enzyme Assays / methods*
Humans
Hydrogen-Ion Concentration
Indicators and Reagents
Matrix Metalloproteinase Inhibitors / metabolism
Metals / metabolism
Temperature

Substances

Indicators and Reagents
Matrix Metalloproteinase Inhibitors
Metals
Collagen
Collagenases