Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies

J Med Virol. 2017 Nov;89(11):2047-2050. doi: 10.1002/jmv.24874. Epub 2017 Jul 6.

Abstract

Background: Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter-laboratory reproducibility of total HIV-1-DNA quantification.

Methods: Ten laboratories of the ANRS-AC11 working group participated by quantifying HIV-DNA with a real-time qPCR assay (Biocentric) in four samples (QCMD).

Results: Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1<QC2<QC3).

Conclusions: Results of this QC validate the feasibility of multicenter studies using this standardized assay.

Keywords: HIV-1 DNA; interlaboratory quality control; quantification.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clinical Laboratory Techniques / methods
  • Clinical Laboratory Techniques / standards*
  • DNA, Viral / analysis*
  • Disease Reservoirs / virology
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • HIV-1 / physiology*
  • Humans
  • Intersectoral Collaboration
  • Quality Control*
  • RNA, Viral / blood
  • Real-Time Polymerase Chain Reaction / methods
  • Real-Time Polymerase Chain Reaction / standards*
  • Reproducibility of Results
  • Viral Load / standards*

Substances

  • DNA, Viral
  • RNA, Viral