Fate and adverse effects of hexabromocyclododecane diastereoisomers (HBCDDs) in a soil-ryegrass pot system

This study explored the fate and adverse effects of 3 main hexabromocyclododecane diastereoisomers (α-, β-, and γ-HBCDDs) in a soil pot system planted with ryegrass (Lolium perenne L.) using a short-term (8 weeks) experiment. At the end of the experiment, soil urease activity in planted spiked soil increased and catalase activity decreased; while there was no obvious change in sucrase and peroxidase activities. HBCDDs mainly accumulated in the root of ryegrass, with root concentration factors (RCF) in the range of 1.46-4.43 and only a small part was transferred to the stem (SCF: 0.198-0.305) and leaf (LCF: 0.042-0.062). The concentration factors varied for different HBCDD diastereoisomers, being in the order of α- > β- > γ-HBCDD for all tissues, indicating preferential accumulation of α-HBCDD in ryegrass tissues. Moreover, the enantiomeric analysis revealed an enrichment of (+)-α-, (-)-β- and (+)-γ-HBCDD enantiomers in ryegrass tissues. β- and γ-HBCDDs (up to 1.90% and 4.11%, respectively) were transformed to aα-HBCDD in ryegrass, while no isomerization product from α-HBCDD was found. Hydroxylated HBCDDs metabolites, such as monoOHHBCDDs and diOHHBCDDs were found in ryegrass tissues for the first time.