Application of C8 liquid chromatography-tandem mass spectrometry for the analysis of enniatins and bassianolides

Justin B Renaud; Megan J Kelman; David R McMullin; Ken K-C Yeung; Mark W Sumarah

doi:10.1016/j.chroma.2017.05.070

Application of C8 liquid chromatography-tandem mass spectrometry for the analysis of enniatins and bassianolides

J Chromatogr A. 2017 Jul 28:1508:65-72. doi: 10.1016/j.chroma.2017.05.070. Epub 2017 Jun 3.

Authors

Justin B Renaud¹, Megan J Kelman², David R McMullin³, Ken K-C Yeung⁴, Mark W Sumarah⁵

Affiliations

¹ London Research and Development Center, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada.
² London Research and Development Center, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada; Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7, Canada.
³ Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, Charlottetown, PEI, C1A 4N6, Canada.
⁴ Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7, Canada; Department of Biochemistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
⁵ London Research and Development Center, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada; Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7, Canada. Electronic address: [email protected].

PMID: 28619584
DOI: 10.1016/j.chroma.2017.05.070

Abstract

Several moderately pathogenic Fusarium species produce enniatins, a family of cyclohexadepsipeptides with insecticidal and phytotoxic activities. A semi-targeted LC-MS/MS approach utilizing reversed-phase C8 UHPLC column chromatography that combines both product ion and neutral loss filtering was developed to detect enniatins and structurally-related compounds in a sample. Using this methodology, resolution of all major enniatins was achieved with a 6-min LC run. From extracts of F. avenaceum, F. acuminatum and F. subglutinans, 23 enniatins and 16 previously unreported bassianolide-like cyclooctadepsipeptides were detected. Enniatins B, B1, A1 and A were the most frequently detected enniatins from silage and inoculated maize. The newly discovered bassianolide compounds were also detected, in lesser amounts on inoculated maize and in naturally contaminated silage.

Keywords: Bassianolide; Emerging mycotoxins; Enniatin; LC–MS/MS; Neutral loss filtering; Product ion filtering.

Publication types

Evaluation Study

MeSH terms

Chromatography, Liquid / methods*
Depsipeptides / chemistry*
Depsipeptides / metabolism
Fusarium / chemistry*
Fusarium / metabolism
Mass Spectrometry
Peptides, Cyclic / chemistry*
Peptides, Cyclic / metabolism
Tandem Mass Spectrometry / methods*
Zea mays / chemistry
Zea mays / microbiology

Substances

Depsipeptides
Peptides, Cyclic
enniatins
bassianolide