Response to Comment on "Xist recruits the X chromosome to the nuclear lamina to enable chromosome-wide silencing"

Chun-Kan Chen; Amy Chow; Mason Lai; Mitchell Guttman

doi:10.1126/science.aam5439

Response to Comment on "Xist recruits the X chromosome to the nuclear lamina to enable chromosome-wide silencing"

Science. 2017 Jun 16;356(6343):eaam5439. doi: 10.1126/science.aam5439.

Authors

Chun-Kan Chen¹, Amy Chow¹, Mason Lai¹, Mitchell Guttman²

Affiliations

¹ Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
² Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. [email protected].

PMID: 28619888
DOI: 10.1126/science.aam5439

Abstract

Wang et al question whether Lamin B receptor is required for Xist-mediated silencing because they claim that our cells contain an inversion rather than a deletion. We present evidence that these cells contain a proper deletion and that the confusion is caused by DNA probes used in the experiment. Accordingly, the points raised have no effect on the conclusions in our paper.

Publication types

Comment

MeSH terms

Gene Silencing
Humans
Nuclear Lamina*
RNA, Long Noncoding / genetics
RNA, Untranslated / genetics
X Chromosome
X Chromosome Inactivation*

Substances

RNA, Long Noncoding
RNA, Untranslated