Absence of Batf3 results in reduced liver pathology in mice infected with Schistosoma japonicum

Parasit Vectors. 2017 Jun 24;10(1):306. doi: 10.1186/s13071-017-2250-1.

Abstract

Background: The involvement of CD8+T cells in schistosomiasis is being increasingly appreciated, but the underlying mechanism is not well defined.

Results: In this study, we showed that the absence of Batf3 alleviated liver damage in Batf3 -/- mice infected with S. japonicum. We found alleviated liver granulomatous inflammation in Batf3 -/- mice with schistosomiasis japonica could not be attributed to the difference in schistosome egg or worm burden. The stronger Tc1 cell responses observed in Batf3 -/- mice suggested that the deletion of Batf3 resulted in more activation of CD8+T cells unexpectedly during the natural infection of schistosomes. We detected a small amount of CD8α+ DCs in the spleen of Batf3 -/- mice at 9w post-infection. This small amount of newly generated CD8α+ DCs might contribute to enhanced activation of CD8+T cells via cross-presentation and activation which then attenuate hepatic pathological damage found in Batf3 -/- mice.

Conclusions: Our study provides evidence that Batf3 is associated with the immunoregulation of the liver granuloma formation, which may confer a new options for schistosomiasis treatment.

Keywords: Batf3; CD8+T cells; CD8α+; Dendritic cells; Helminth; Schistosoma japonicum.

MeSH terms

  • Animals
  • Basic-Leucine Zipper Transcription Factors / chemistry
  • Basic-Leucine Zipper Transcription Factors / genetics
  • Basic-Leucine Zipper Transcription Factors / immunology
  • Basic-Leucine Zipper Transcription Factors / physiology*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • Dendritic Cells / immunology
  • Female
  • Flow Cytometry
  • Granuloma / immunology
  • Granuloma / parasitology
  • Granuloma / pathology*
  • Liver / parasitology
  • Liver / pathology*
  • Liver Diseases / immunology
  • Liver Diseases / parasitology
  • Liver Diseases / pathology*
  • Mice
  • Mice, Inbred C57BL
  • Real-Time Polymerase Chain Reaction
  • Repressor Proteins / chemistry
  • Repressor Proteins / genetics
  • Repressor Proteins / immunology
  • Repressor Proteins / physiology*
  • Schistosomiasis japonica / pathology*
  • Snails / parasitology
  • Spleen / cytology
  • Spleen / immunology
  • Th1 Cells / cytology
  • Th1 Cells / immunology

Substances

  • Basic-Leucine Zipper Transcription Factors
  • Repressor Proteins
  • SNFT protein, mouse