Objective: To investigate the expression of cAMP-dependent protein kinase inhibitor beta (PKIB) in colorectal carcinoma (CRC) and its association with the clinicopathological factors of the patients.
Methods: The expression of PKIB mRNA was detected with quantitative real-time PCR in 34 CRC tissues and paired adjacent tissues. Immunohistochemistry was used to detect the expression of PKIB protein in 72 CRC tissue specimens, and the relationship between PKIB protein expression and the clinicopathological features of the patients was analyzed.
Results: The expression of PKIB mRNA was significantly higher in CRC tissues than in the paired asjacent tissues (P<0.0001). The expression of PKIB protein in CRC patients was closely related with tumor infiltration (T stage) (P=0.038) but not with age, gender, tumor size, location, lymph node metastasis (N stage) or distant metastasis (M stage) (P>0.05). The survival time of patients with high PKIB expressions was significantly shorter than that of patients with low PKIB expressions (70.532∓6.190 vs 93.500∓5.847 months, P=0.023).
Conclusion: A high expression of PKIB in CRC is positively correlated with tumor infiltration (T stage) and a poor prognosis, suggesting an important role of PKIB in the development of CRC and its value as an indicator for prognostic evaluation of CRC patients.
目的: 探讨结直肠癌组织中cAMP依赖型蛋白激酶抑制剂β（PKIB）的表达水平及其与结直肠癌患者临床病理因素之间的关系。
方法: 利用实时荧光定量PCR法检测34对结直肠癌组织和配对癌旁正常组织中PKIB的表达水平，应用免疫组织化学法对72例结直肠癌组织中PKIB的表达水平进行检测，并分析PKIB表达的临床意义。
结果: 在结直肠癌组织中PKIB mRNA及蛋白的表达水平均明显高于配对癌旁正常组织（P < 0.0001）。PKIB蛋白的表达水平与结直肠癌患者T分期正相关（P=0.038），而与患者的年龄、性别、肿瘤大小、发病部位以及淋巴结转移（N分期）、远隔器官转移（M分期）无明显相关性（P>0.05）。高表达PKIB的结直肠癌患者生存时间明显短于低表达的患者（70.532±6.190 vs 93.500±5.847，P=0.023）。
结论: PKIB在结直肠癌组织中高表达，其高表达与结直肠癌患者T分期及预后不良呈正相关，提示PKIB可能在结直肠癌演进中发挥了重要作用并有望成为一种新的结直肠癌预后判断标志物。