Discovery of Novel Potent Reversible and Irreversible Myeloperoxidase Inhibitors Using Virtual Screening Procedure

J Med Chem. 2017 Aug 10;60(15):6563-6586. doi: 10.1021/acs.jmedchem.7b00285. Epub 2017 Jul 19.

Abstract

The heme enzyme myeloperoxidase (MPO) participates in innate immune defense mechanism through formation of microbicidal reactive oxidants. However, evidence has emerged that MPO-derived oxidants contribute to propagation of inflammatory diseases. Because of the deleterious effects of circulating MPO, there is a great interest in the development of new efficient and specific inhibitors. Here, we have performed a novel virtual screening procedure, depending on ligand-based pharmacophore modeling followed by structure-based virtual screening. Starting from a set of 727842 compounds, 28 molecules were selected by this virtual method and tested on MPO in vitro. Twelve out of 28 compounds were found to have an IC50 less than 5 μM. The best inhibitors were 2-(7-methoxy-4-methylquinazolin-2-yl)guanidine (28) and (R)-2-(1-((2,3-dihydro-1H-imidazol-2-yl)methyl)pyrrolidin-3-yl)-5-fluoro-1H-benzo[d]imidazole (42) with IC50 values of 44 and 50 nM, respectively. Studies on the mechanism of inhibition suggest that 28 is the first potent mechanism-based inhibitor and inhibits irreversibly MPO at nanomolar concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzimidazoles / chemical synthesis
  • Benzimidazoles / pharmacology*
  • Benzimidazoles / toxicity
  • Cell Line
  • Databases, Chemical
  • Drug Design
  • Drug Evaluation, Preclinical
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / pharmacology*
  • Enzyme Inhibitors / toxicity
  • Glutamic Acid / chemistry
  • Glutamine / chemistry
  • Guanidines / chemical synthesis
  • Guanidines / pharmacology*
  • Guanidines / toxicity
  • Humans
  • Hydrogen Peroxide / chemistry
  • Kinetics
  • Lactoperoxidase / antagonists & inhibitors
  • Lipoproteins, LDL / chemistry
  • Models, Chemical
  • Molecular Docking Simulation
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Oxidation-Reduction
  • Peroxidase / antagonists & inhibitors*
  • Quinazolines / chemical synthesis
  • Quinazolines / pharmacology*
  • Quinazolines / toxicity
  • Stereoisomerism

Substances

  • 2-(1-((2,3-dihydro-1H-imidazol-2-yl)methyl)pyrrolidin-3-yl)-5-fluoro-1H-benzo(d)imidazole
  • 2-(7-methoxy-4-methylquinazolin-2-yl)guanidine
  • Benzimidazoles
  • Enzyme Inhibitors
  • Guanidines
  • Lipoproteins, LDL
  • Quinazolines
  • Glutamine
  • Glutamic Acid
  • Hydrogen Peroxide
  • Lactoperoxidase
  • Peroxidase