Development of a real-time PCR method for quantification of Prevotella histicola from the gut

Baskar Balakrishnan; David Luckey; Eric Marietta; Melissa Karau; Robin Patel; Joseph Murray; Veena Taneja

doi:10.1016/j.anaerobe.2017.06.019

Development of a real-time PCR method for quantification of Prevotella histicola from the gut

Anaerobe. 2017 Dec:48:37-41. doi: 10.1016/j.anaerobe.2017.06.019. Epub 2017 Jul 3.

Authors

Baskar Balakrishnan¹, David Luckey¹, Eric Marietta², Melissa Karau³, Robin Patel³, Joseph Murray², Veena Taneja⁴

Affiliations

¹ Department of Immunology, Mayo Clinic, Rochester, MN55905, USA.
² Department of Gastroenterology, Mayo Clinic, Rochester, MN55905, USA.
³ Department of Clinical Microbiology, Mayo Clinic, Rochester, MN55905, USA.
⁴ Department of Immunology, Mayo Clinic, Rochester, MN55905, USA. Electronic address: [email protected].

PMID: 28684342
DOI: 10.1016/j.anaerobe.2017.06.019

Abstract

We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 10³ colony-forming units (CFU) in the gut.

Keywords: Anaerobe; Gut microbiome; Prevotella histicola; Real-time PCR quantification; qPCR.

MeSH terms

Animals
Bacteroidaceae Infections / diagnosis*
Bacteroidaceae Infections / microbiology
DNA Primers / genetics
DNA, Bacterial / genetics
Intestines / microbiology*
Mice
Mice, Transgenic
Prevotella / isolation & purification*
RNA, Ribosomal, 16S / genetics
Real-Time Polymerase Chain Reaction / methods*

Substances

DNA Primers
DNA, Bacterial
RNA, Ribosomal, 16S