Novel Genetic Findings in a Chinese Family with Axenfeld-Rieger Syndrome

Kuanshu Li; Liu Yang; Ying Liu; Ding Lin

doi:10.1155/2017/5078079

Novel Genetic Findings in a Chinese Family with Axenfeld-Rieger Syndrome

J Ophthalmol. 2017:2017:5078079. doi: 10.1155/2017/5078079. Epub 2017 Jun 13.

Authors

Kuanshu Li^{1

2}, Liu Yang^{1

2}, Ying Liu², Ding Lin^{1

2}

Affiliations

¹ Aier School of Ophthalmology, Central South University, Changsha, China.
² Aier Eye Hospital, Changsha, Hunan, China.

Abstract

Purpose: To describe a Chinese family with Axenfeld-Rieger syndrome (ARS) and report our novel genetic findings.

Methods: Nine members of the same family underwent complete ophthalmologic examinations and genetic analysis. Genomic DNA was isolated from veinal blood and amplifed using PCR; the products of PCR were sequenced and compared with FOXC1 and PITX2 genes, from which the mutations were found.

Results: Through the ophthalmologic examinations, 8 subjects were diagnosed as ARS and 1 subject was normal. A homozygous mutation c.1139_1141dupGCG(p.Gly380_Ala381insGly) and a heterozygous mutation c.1359_1361dupCGG(p.Gly456_Gln457insGly) in FOXC1 were identified in all subjects. The mutation (c.-10-30T>C) was identified in PITX2 in subjects III-1 and III-3.

Conclusions: We found novel gene mutations in a Chinese family with ARS, which provides us with a better understanding of the gene mutation spectrum of ARS and the assistance for the genetic counseling and gene-specific therapy in the future.