LC/MS/MS Profiling of Tissue Oxysterols and its Application in Dextran Sodium Sulphate Induced Mouse Colitis Models

Curr Top Med Chem. 2017;17(24):2781-2790. doi: 10.2174/1568026617666170713165519.

Abstract

We have developed a workflow to extract, separate, and semi-quantify bioactive oxysterols from mouse colon tissues and fecal matters using solid- and liquid-phase extractions, enzymatic and chemical modifications, and stable-isotope dilution LC/MS/MS. The method was applied to a dextran sodium sulphate (DSS)-induced mouse colitis model, which revealed that one particular dihydroxycholesterol (diOHC), 7α,25-diOHC, was significantly elevated in both colon tissue and fecal matters of mice with colitis compared to that in naïve mice. The extent of 7α,25-diOHC elevation was positively correlated with colitis severity.

Keywords: Dihydroxycholesterols; Mouse colitis biomarker.; Oxysterol analysis; Oxysterol quantification; Oxysterol separation.

MeSH terms

  • Animals
  • Chromatography, Liquid
  • Colitis / chemically induced*
  • Colon / chemistry*
  • Colon / pathology
  • Dextran Sulfate
  • Disease Models, Animal*
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Oxysterols / chemistry
  • Oxysterols / isolation & purification*
  • Tandem Mass Spectrometry

Substances

  • Oxysterols
  • Dextran Sulfate