Occurrence and clonal diversity of multidrug-resistant Klebsiella pneumoniae recovered from inanimate surfaces in Algerian hospital environment: First report of armA, qnrB and aac(6')-Ib-cr genes

Karima Zenati; Farida Sahli; Vincent Garcia; Sofiane Bakour; Djellali Belhadi; Jean Marc Rolain; Abdelaziz Touati

doi:10.1016/j.jgar.2017.05.015

Occurrence and clonal diversity of multidrug-resistant Klebsiella pneumoniae recovered from inanimate surfaces in Algerian hospital environment: First report of armA, qnrB and aac(6')-Ib-cr genes

J Glob Antimicrob Resist. 2017 Sep:10:148-153. doi: 10.1016/j.jgar.2017.05.015. Epub 2017 Jul 19.

Authors

Karima Zenati¹, Farida Sahli², Vincent Garcia³, Sofiane Bakour³, Djellali Belhadi⁴, Jean Marc Rolain³, Abdelaziz Touati⁵

Affiliations

¹ Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria; Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
² Laboratoire de Microbiologie, CHU de Setif, Algeria.
³ Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
⁴ Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria.
⁵ Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria. Electronic address: [email protected].

PMID: 28732788
DOI: 10.1016/j.jgar.2017.05.015

Abstract

Purpose: The aim of this study is to characterize the molecular support of antibiotic resistance in MDR Klebsiella pneumoniae recovered from inanimate surfaces between March 2012 to February 2014 in three teaching hospitals (Setif, Bejaia and Constantine) in Algeria.

Results: Forty-four K. pneumoniae producing ESBL were detected and bla_CTX-M-15 and bla_CTX-M-3 were detected respectively in 41 and 3 isolates. These K. pneumoniae isolates producing ESBL were also resistant to gentamicin (87%), tobramicin (87%), ciprofloxacin (66%) and ofloxacin (62%). Aminoglycosides resistance genes detected were 16S rRNA methylase (armA), aminoglycoside acetyl-transferase (aac(6')-Ib), aminoglycoside nucleotidyl-transferase (aadA2) and aminoglycoside, phosphoryl-transferase (ant3″Ih-aac(6')-IId). Plasmid-mediated quinolone resistance (PMQR) genes detected were aac(6')-Ib-cr (34 isolates) and qnrB genes in (34 isolates). Multilocus sequence typing (MLST) resulted in 12 different sequence types (STs) regrouped into 5 clonal complexes (CC147, CC17, CC37, CC2 and CC23), one clonal group (CG485) and 4 singletons (ST1426, ST405, ST1308, ST873).

Conclusion: Here, we report the detection of the ESBLs encoding gene linked with plasmid-mediated quinolone resistance (PMQR) and aminoglycosides resistance recovered from inanimate surfaces in hospital environment.

Keywords: ESBLs; Hospital environment; Inanimate surfaces; Klebsiella pneumoniae; PMQR; armA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyltransferases / genetics
Algeria / epidemiology
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Drug Resistance, Multiple, Bacterial / drug effects
Drug Resistance, Multiple, Bacterial / genetics*
Hospitals
Humans
Klebsiella Infections / epidemiology
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics*
Methyltransferases / genetics
Microbial Sensitivity Tests
Molecular Epidemiology
Molecular Typing
Multilocus Sequence Typing
Plasmids
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Qnr protein, Klebsiella pneumoniae
Methyltransferases
rRNA (adenosine-O-2'-)methyltransferase
Acetyltransferases
aminoglycoside acetyltransferase
beta-lactamase CTX-M-15
beta-lactamase CTX-M-3
beta-Lactamases