Abstract
We studied the properties of a tissue-engineered trachea consisting of a polyethylene terephthalate scaffold populated with autologous bone marrow mononuclear cells. The tissue-engineered constructs were obtained before surgery, during the postoperative period, and during autopsy. Cytomorphological analysis during the postoperative period showed the presence of mesenchymal stem cells on the inner surface of the implant on day 3 after surgery and cells of the respiratory epithelium on day 10-14. In autopsy samples, single epithelial cells, endothelial cells, and basal cells were found. Biocompatibility of the tissue-engineered trachea with autologous mononuclear cells of the patient was demonstrated.
Keywords:
biocompatibility; mononuclear cells; synthetic scaffold; tissue-engineering construct of the trachea.
MeSH terms
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Antigens, CD34 / genetics
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Antigens, CD34 / metabolism
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Biocompatible Materials / chemical synthesis
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Biocompatible Materials / pharmacology*
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Biomarkers / metabolism
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Bone Marrow Cells / cytology
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Bone Marrow Cells / metabolism
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Bronchoalveolar Lavage Fluid / chemistry
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Bronchoalveolar Lavage Fluid / cytology
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Cell Adhesion / drug effects
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Gene Expression
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Humans
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Keratin-7 / genetics
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Keratin-7 / metabolism
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Ki-67 Antigen / genetics
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Ki-67 Antigen / metabolism
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / metabolism
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Leukocytes, Mononuclear / transplantation
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Polyethylene Terephthalates / chemical synthesis
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Polyethylene Terephthalates / pharmacology*
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Tissue Engineering
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Tissue Scaffolds*
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Trachea / surgery*
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Transplantation, Autologous
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Vimentin / genetics
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Vimentin / metabolism
Substances
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Antigens, CD34
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Biocompatible Materials
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Biomarkers
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CKAP4 protein, human
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Keratin-7
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Ki-67 Antigen
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Membrane Proteins
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Polyethylene Terephthalates
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Vimentin