Evaluation of Taterapox Virus in Small Animals

Viruses. 2017 Aug 1;9(8):203. doi: 10.3390/v9080203.

Abstract

Taterapox virus (TATV), which was isolated from an African gerbil (Tatera kempi) in 1975, is the most closely related virus to variola; however, only the original report has examined its virology. We have evaluated the tropism of TATV in vivo in small animals. We found that TATV does not infect Graphiurus kelleni, a species of African dormouse, but does induce seroconversion in the Mongolian gerbil (Meriones unguiculatus) and in mice; however, in wild-type mice and gerbils, the virus produces an unapparent infection. Following intranasal and footpad inoculations with 1 × 10⁶ plaque forming units (PFU) of TATV, immunocompromised stat1-/- mice showed signs of disease but did not die; however, SCID mice were susceptible to intranasal and footpad infections with 100% mortality observed by Day 35 and Day 54, respectively. We show that death is unlikely to be a result of the virus mutating to have increased virulence and that SCID mice are capable of transmitting TATV to C57BL/6 and C57BL/6 stat1-/- animals; however, transmission did not occur from TATV inoculated wild-type or stat1-/- mice. Comparisons with ectromelia (the etiological agent of mousepox) suggest that TATV behaves differently both at the site of inoculation and in the immune response that it triggers.

Keywords: SCID; footpad; intranasal; monkeypox; orthopoxvirus; smallpox; taterapox; variola.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antiviral Agents / therapeutic use
  • Disease Models, Animal
  • Ectromelia virus / genetics
  • Ectromelia virus / physiology
  • Ectromelia, Infectious / virology
  • Host Specificity
  • Mice
  • Mice, Inbred C57BL
  • Mice, SCID
  • Orthopoxvirus / genetics
  • Orthopoxvirus / immunology
  • Orthopoxvirus / isolation & purification
  • Orthopoxvirus / physiology*
  • Poxviridae Infections / drug therapy
  • Poxviridae Infections / immunology
  • Poxviridae Infections / transmission
  • Poxviridae Infections / virology*
  • STAT1 Transcription Factor / deficiency
  • STAT1 Transcription Factor / genetics
  • Viral Tropism*

Substances

  • Antiviral Agents
  • STAT1 Transcription Factor
  • Stat1 protein, mouse