An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay

Food Chem. 2017 Dec 15:237:290-296. doi: 10.1016/j.foodchem.2017.05.115. Epub 2017 May 23.

Abstract

An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0-3.0μgkg-1 and 4.0-10.0μgkg-1, respectively. The average recoveries of MQCA that was spiked into samples at concentrations of 4.0-100μgkg-1 were 80.1-87.7%, with relative standard deviations of less than 8.5%. Using the GICA method, the limits of detection for MQCA in different samples were 10μgkg-1 and 50μgkg-1. No false negatives or false positives were observed.

Keywords: 3-Methyl-quinoxaline-2-carboxylic acid; Colloidal gold-based immunochromatographic assay; Edible tissues; High-performance liquid chromatography with ultraviolet detection; Immunoaffinity column purification.

MeSH terms

  • Animals
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Gold Colloid
  • Muscles
  • Quinoxalines / analysis*
  • Swine

Substances

  • Gold Colloid
  • Quinoxalines
  • quinoxaline-2-carboxylic acid