Abstract
With hepatocytes in suspension, freshly isolated from meal-fed rats, no significant effect of ionomycin on the rate of de novo fatty acid synthesis was observed, whereas phorbol myristate acetate (PMA) was strongly stimulatory. The combination of ionomycin and PMA produced the same stimulation as was seen with PMA alone. Stimulation of fatty acid synthesis by vasopressin was comparable and not additive to that observed with PMA, indicating that activation of protein kinase C is solely responsible for this metabolic effect of vasopressin. Both vasopressin and PMA increased acetyl-CoA carboxylase activity in isolated rat hepatocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl-CoA Carboxylase / metabolism
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Animals
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Calcium Chloride / pharmacology
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Chlorides / pharmacology
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Drug Synergism
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Ethers / pharmacology
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Fatty Acids / biosynthesis*
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Gallic Acid / analogs & derivatives
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Gallic Acid / pharmacology
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Ionomycin
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Lithium / pharmacology
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Lithium Chloride
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Liver / drug effects*
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Liver / enzymology
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Male
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Protein Kinase C / metabolism
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Rats
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Rats, Inbred Strains
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Tetradecanoylphorbol Acetate / pharmacology*
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Type C Phospholipases / metabolism
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Vasopressins / pharmacology
Substances
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Chlorides
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Ethers
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Fatty Acids
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Vasopressins
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Ionomycin
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8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate
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Gallic Acid
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Lithium
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Protein Kinase C
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Type C Phospholipases
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Acetyl-CoA Carboxylase
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Lithium Chloride
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Calcium Chloride
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Tetradecanoylphorbol Acetate