Abstract
To prevent tumorigenesis, p53 stimulates transcription by facilitating the recruitment of the transcription machinery on target gene promoters. Cryo-Electron Microscopy studies on p53-bound RNA Polymerase II (Pol II) reveal that p53 structurally regulates Pol II to affect its DNA binding and elongation, providing new insights into p53-mediated transcriptional regulation.
Keywords:
3D structure; RNA polymerase II; cryo-electron microscopy; p53; transcription activation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinogenesis / genetics
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Carcinogenesis / metabolism
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Cryoelectron Microscopy
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Humans
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Models, Molecular
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Promoter Regions, Genetic
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Protein Binding
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Protein Conformation
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Protein Interaction Maps
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RNA Polymerase II / chemistry
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RNA Polymerase II / metabolism*
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RNA Polymerase II / ultrastructure
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Transcriptional Activation*
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Tumor Suppressor Protein p53 / chemistry
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Tumor Suppressor Protein p53 / metabolism*
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Tumor Suppressor Protein p53 / ultrastructure
Substances
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Tumor Suppressor Protein p53
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RNA Polymerase II