Objective: To investigate the changes in the percentages and balance of CD4+T cell subsets including T helper cells (Thl, Th2, and Thl7) and T regulatory cells (Treg) in patients with ovarian cancer.
Methods: Peripheral blood samples were collected from 30 patients with ovarian cancer and 20 healthy subjects for analysis of the percentages of Thl, Th2, Thl7 and Treg using flow cytometry.
Results: Compared with the control subjects, the patients with ovarian cancer showed significantly increased percentages of Th2, Thl7 and Treg (P<0.05) but significantly decreased percentage of Th1 in the peripheral blood of patients with ovarian cancer (P<0.05). The changes in CD4+ T cell subsets were significantly correlated with the clinical stage of the tumor (P<0.05) but not with the histological type or cell differentiation (P>0.05). The Th1/Th2 ratio was significantly decreased in ovarian cancer patients (P<0.05) with obvious Th2 polarization compared with control group. The Treg/Th17 ratio was significantly increased in ovarian cancer patients (P<0.05).
Conclusion: Patients with in ovarian cancer have abnormal expressions of CD4+T cell subsets in the peripheral blood with Th1/Th2 and Treg/Th17 imbalance, and these findings provide evidence for clinical immunotherapy of ovarian cancer.
目的: 探讨卵巢癌患者外周血中CD4+T细胞亚群：辅助T细胞（Th，包括Th1、Th2、Th17）及调节性T淋巴细胞（Treg）表达、平衡关系的变化，以及在卵巢癌发病机制中的作用。
方法: 流式细胞术检测30例卵巢癌疾病组及20例健康对照组外周血中Thl、Th2、Thl7、Treg表达占CD4+T细胞的百分率，并计算Th1/Th2、Th17/Treg的比值。
结果: 卵巢癌患者外周血中Th2、Th17及Treg亚群占CD4+T细胞的百分率明显高于健康对照组（P < 0.05），而Th1百分率低于健康对照组（P < 0.05），各亚群变化与临床分期相关，但与组织类型及细胞分化无关（P > 0.05）。卵巢癌组Th1/Th2比值降低，与对照组比较有统计学意义（P < 0.05），卵巢癌患者存在Th2方向极化；卵巢癌组Treg/Th17比值升高，与对照组比较有统计学意义（P < 0.05）。
结论: 卵巢癌患者外周血中存在CD4+细胞亚群表达异常，存在Th1/Th2、Treg/Th17比值失衡，为卵巢癌临床免疫治疗提供理论支持