A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells

Chao Huang; Zhongkai Zhu; Xiyue Cao; Xingfu Chen; Yuping Fu; Zhengli Chen; Lixia Li; Xu Song; Renyong Jia; Zhongqiong Yin; Gang Ye; Bin Feng; Yuanfeng Zou

doi:10.3390/molecules22081341

A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells

Molecules. 2017 Aug 12;22(8):1341. doi: 10.3390/molecules22081341.

Authors

Chao Huang^{1

2}, Zhongkai Zhu³, Xiyue Cao^{4

5}, Xingfu Chen⁶, Yuping Fu^{7

8}, Zhengli Chen^{9

10}, Lixia Li^{11

12}, Xu Song^{13

14}, Renyong Jia¹⁵, Zhongqiong Yin^{16

17}, Gang Ye¹⁸, Bin Feng¹⁹, Yuanfeng Zou^{20

21}

Affiliations

¹ Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
² Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
³ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
⁴ Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
⁵ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
⁶ Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
⁷ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
⁸ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
⁹ Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁰ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹¹ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹² Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹³ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁴ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁵ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁶ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁷ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁸ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
¹⁹ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
²⁰ Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].
²¹ Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. [email protected].

Abstract

Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1α, which would be valuable for further research and applications.

Keywords: SJZD; Sijunzi Decoction; antioxidant defense; oxidative stress; pectic polysaccharide.

MeSH terms

Antioxidants / chemistry*
Antioxidants / pharmacology
Cell Culture Techniques
Cell Line
Cell Survival
Drugs, Chinese Herbal / chemistry*
Drugs, Chinese Herbal / pharmacology
Gastrointestinal Diseases / drug therapy
Humans
Magnetic Resonance Spectroscopy / methods
Molecular Conformation
Molecular Structure
Pectins / chemistry*
Polysaccharides / chemistry*
Polysaccharides / pharmacology
Spectroscopy, Fourier Transform Infrared / methods
Spleen / drug effects
Structure-Activity Relationship

Substances

Antioxidants
Drugs, Chinese Herbal
Polysaccharides
rhamnogalacturonan I
Pectins
polygalacturonic acid