Abstract
The RareCyte CyteFinder instrument is an automated scanner that allows rapid identification of circulating tumor cells (CTCs) on microscope slides prepared by the AccuCyte process (see Chapter 13 ) and stained by immunofluorescence. Here, we present the workflow for CyteFinder scanning, analysis, and CyteMapper scan review which includes CTC confirmation and report generation.
Keywords:
Circulating tumor cells; Fluorescence scanning; Image analysis; Scoring algorithm.
MeSH terms
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / metabolism
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Automation, Laboratory / instrumentation
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / immunology
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Biomarkers, Tumor / metabolism
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Cell Count
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Cell Line, Tumor
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Cell Separation / instrumentation
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Cell Separation / methods*
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Cells, Immobilized / immunology
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Cells, Immobilized / metabolism
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Cells, Immobilized / pathology*
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Centrifugation / instrumentation
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Centrifugation / methods
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Epithelial Cell Adhesion Molecule / genetics
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Epithelial Cell Adhesion Molecule / immunology
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Epithelial Cell Adhesion Molecule / metabolism
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Equipment Design
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Fluorescent Antibody Technique / methods
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Fluorescent Dyes / chemistry
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Humans
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Image Processing, Computer-Assisted / methods*
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Immunoconjugates / chemistry
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Keratins / genetics
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Keratins / immunology
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Keratins / metabolism
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Leukocyte Common Antigens / genetics
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Leukocyte Common Antigens / immunology
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Leukocyte Common Antigens / metabolism
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Neoplasms / blood
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Neoplasms / diagnosis*
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Neoplasms / immunology
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Neoplasms / pathology
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Neoplastic Cells, Circulating / immunology
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Neoplastic Cells, Circulating / metabolism
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Neoplastic Cells, Circulating / pathology*
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Protein Binding
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Single-Cell Analysis / instrumentation
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Single-Cell Analysis / methods*
Substances
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Antibodies, Monoclonal
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Biomarkers, Tumor
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EPCAM protein, human
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Epithelial Cell Adhesion Molecule
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Fluorescent Dyes
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Immunoconjugates
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Keratins
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Leukocyte Common Antigens
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PTPRC protein, human