Multiple genetic variants associated with posttransplantation diabetes mellitus in Chinese Han populations

J Clin Lab Anal. 2018 Mar;32(3):e22308. doi: 10.1002/jcla.22308. Epub 2017 Aug 23.

Abstract

Objectives: Posttransplantation diabetes mellitus (PTDM) is a major complication after solid organ transplantation. This study is to investigate the association of nine genetic variant factors and PTDM in Chinese Han patients.

Methods: HLA-DP (rs3077, rs9277535), HLA-DQ (rs7453920), signal transducer and activator of transcription 4 (STAT4) (rs7574865), IL-28B (rs12979860, rs8099917, and rs12980275), and IL-18 (rs1946518 and rs187238) were investigated in 260 liver transplant recipients (PTDM vs non-PTDM) by high-resolution melting curve analysis. Serum interleukin (IL)-1β, IL-6, IL-8, IL-17, interferon-γ, inducible protein-10, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1b were analyzed by a Bio-Plex suspension array system (Bio-Plex Multiplex Immunoassays, Bio-Rad, Hercules, CA, USA).

Results: Signal transducer and activator of transcription 4 (rs7574865) T allele and IL-18 (rs1946518) A allele increase the risk for insulin resistance and PTDM.

Conclusions: Recipients with STAT4 (rs7574865) T allele are associated with an increased concentration of IL-1β, interferon-γ, monocyte chemoattractant protein, and macrophage inflammatory protein-1b. The genetic variants of STAT4 (rs7574865) and IL-18 (rs1946518) may be new important markers for PTDM.

Keywords: PTDM; HLA-DP; HLA-DQ; IL-18; IL-28B; STAT4.

MeSH terms

  • Adult
  • Asian People* / genetics
  • Asian People* / statistics & numerical data
  • China / epidemiology
  • Diabetes Mellitus* / epidemiology
  • Diabetes Mellitus* / genetics
  • Female
  • Genetic Variation / genetics*
  • HLA-DP Antigens / genetics
  • HLA-DQ Antigens / genetics
  • Humans
  • Insulin Resistance / genetics
  • Interferons
  • Interleukin-18 / genetics
  • Interleukins / genetics
  • Liver Transplantation* / adverse effects
  • Liver Transplantation* / statistics & numerical data
  • Male
  • Middle Aged
  • STAT4 Transcription Factor / genetics

Substances

  • HLA-DP Antigens
  • HLA-DQ Antigens
  • interferon-lambda, human
  • IL18 protein, human
  • Interleukin-18
  • Interleukins
  • STAT4 Transcription Factor
  • STAT4 protein, human
  • Interferons