Renal effects of Bunodosoma caissarum crude extract: Prostaglandin and endothelin involvement

Toxicon. 2017 Nov:138:78-81. doi: 10.1016/j.toxicon.2017.08.019. Epub 2017 Aug 23.

Abstract

Sea anemones contain a variety of interesting biologically active compounds, including some potent toxins. PLA2 from Bunodosoma caissarum, a sea anemone endemic in the Brazilian southern coast, has shown renal alterations on isolated kidney. The aim of this study was to evaluate the renal and vascular effects of B. caissarum crude extract (BcE) on isolated perfused kidney and arteriolar mesenteric bed, as well the involvement of prostaglandins and endothelin. BcE did not show any effect on arteriolar mesenteric bed, but increased perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate and decreased the percentage of sodium tubular transport on isolated perfused kidney. Indomethacin blocked the renal effects induced by BcE and tezosentan only partially blocked these effects. These results demonstrate the effects of BcE on kidney in situ, suggesting the involvement of prostaglandins and endothelin.

Keywords: Bunodosoma caissarum; Endothelin; Envenomation; Isolated perfused kidney; Prostaglandin; Sea anemone.

MeSH terms

  • Animals
  • Cnidarian Venoms / toxicity*
  • Endothelins
  • Glomerular Filtration Rate / drug effects
  • Indomethacin / pharmacology
  • Kidney / drug effects*
  • Male
  • Mesenteric Arteries / drug effects
  • Prostaglandins
  • Pyridines / pharmacology
  • Rats, Wistar
  • Sea Anemones / chemistry*
  • Tetrazoles / pharmacology
  • Vascular Resistance / drug effects

Substances

  • Cnidarian Venoms
  • Endothelins
  • Prostaglandins
  • Pyridines
  • Tetrazoles
  • tezosentan
  • Indomethacin