Rodent-free cyclical transmission of Trypanosoma brucei brucei

Arunasalam Naguleswaran; Isabel Roditi

doi:10.1016/j.molbiopara.2017.08.005

Rodent-free cyclical transmission of Trypanosoma brucei brucei

Mol Biochem Parasitol. 2017 Oct:217:16-18. doi: 10.1016/j.molbiopara.2017.08.005. Epub 2017 Aug 24.

Authors

Arunasalam Naguleswaran¹, Isabel Roditi²

Affiliations

¹ Institute of Cell Biology, Baltzerstrasse 4, CH-3012 Bern, Switzerland. Electronic address: [email protected].
² Institute of Cell Biology, Baltzerstrasse 4, CH-3012 Bern, Switzerland. Electronic address: [email protected].

PMID: 28843782
DOI: 10.1016/j.molbiopara.2017.08.005

Abstract

We provide a simple protocol enabling cyclical transmission of Trypanosoma brucei brucei to be performed without the need for mammals. These procedures have two advantages: they are in line with 3R principles of animal use - replace, refine, reduce - and may enable more laboratories to study the complete life cycle.

Keywords: 3R concept; Animal replacement; Metacyclic forms; Salivary glands; Trypanosome; Tsetse.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Life Cycle Stages
Rodentia / parasitology
Salivary Glands / parasitology
Trypanosoma brucei brucei / physiology*
Trypanosomiasis, African / parasitology*
Trypanosomiasis, African / transmission*
Tsetse Flies / parasitology

Grants and funding

55007650/HHMI/Howard Hughes Medical Institute/United States