A multiplex real-time PCR for the direct, fast, economic and simultaneous detection of the carbapenemase genes blaKPC, blaNDM, blaVIM and blaOXA-48

Daniel Weiß; Ines Engelmann; Sascha D Braun; Stefan Monecke; Ralf Ehricht

doi:10.1016/j.mimet.2017.08.017

A multiplex real-time PCR for the direct, fast, economic and simultaneous detection of the carbapenemase genes blaKPC, blaNDM, blaVIM and blaOXA-48

J Microbiol Methods. 2017 Nov:142:20-26. doi: 10.1016/j.mimet.2017.08.017. Epub 2017 Aug 24.

Authors

Daniel Weiß¹, Ines Engelmann², Sascha D Braun², Stefan Monecke³, Ralf Ehricht²

Affiliations

¹ Alere Technologies GmbH, Jena, Germany; InfectoGnostics Research Campus, Jena, Germany; University Medical Center of Jena, Germany. Electronic address: [email protected].
² Alere Technologies GmbH, Jena, Germany; InfectoGnostics Research Campus, Jena, Germany.
³ Alere Technologies GmbH, Jena, Germany; InfectoGnostics Research Campus, Jena, Germany; Institute for Medical Microbiology and Hygiene, Technische Universität Dresden, Germany.

PMID: 28844721
DOI: 10.1016/j.mimet.2017.08.017

Abstract

A novel multiplex real-time PCR was designed to detect the clinically most important carbapenemase genes, blaKPC, blaVIM, blaNDM and blaOXA-48. The multiplex assay was verified testing genomic DNA of 24 carbapenemase-producing strains. It was validated using a blinded panel of 82 carbapenemase-producing and 50 non-producing isolates by direct colony PCR.

Keywords: CPO; Carbapenemases; Colony PCR; Hospital; Microarray; Multiplex; Real-time PCR.

MeSH terms

Bacterial Proteins / genetics*
Base Sequence
Carbapenem-Resistant Enterobacteriaceae / drug effects*
Carbapenem-Resistant Enterobacteriaceae / genetics*
Carbapenem-Resistant Enterobacteriaceae / isolation & purification
Carbapenems / pharmacology
Genotype
Microbial Sensitivity Tests / methods
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
beta-Lactamases / genetics*

Substances

Bacterial Proteins
Carbapenems
beta-Lactamases
carbapenemase