Roles of increased glycaemic variability, GLP-1 and glucagon in hypoglycaemia after Roux-en-Y gastric bypass

Eur J Endocrinol. 2017 Dec;177(6):455-464. doi: 10.1530/EJE-17-0446. Epub 2017 Aug 30.

Abstract

Objective: Roux-en-Y gastric bypass (RYGB) surgery is currently the most effective treatment for diabetes and obesity. An increasingly recognized and highly disabling complication of RYGB is postprandial hypoglycaemia (PPH). The pathophysiology of PPH remains unclear with multiple mechanisms suggested including nesidioblastosis, altered insulin clearance and increased glucagon-like peptide-1 (GLP-1) secretion. Whilst many PPH patients respond to dietary modification, some have severely disabling symptoms. Multiple treatments are proposed, including dietary modification, GLP-1 antagonism, GLP-1 analogues and even surgical reversal, with none showing a more decided advantage over the others. A greater understanding of the pathophysiology of PPH could guide the development of new therapeutic strategies.

Methods: We studied a cohort of PPH patients at the Imperial Weight Center. We performed continuous glucose monitoring to characterize their altered glycaemic variability. We also performed a mixed meal test (MMT) and measured gut hormone concentrations.

Results: We found increased glycaemic variability in our cohort of PPH patients, specifically a higher mean amplitude glucose excursion (MAGE) score of 4.9. We observed significantly greater and earlier increases in insulin, GLP-1 and glucagon in patients who had hypoglycaemia in response to an MMT (MMT Hypo) relative to those that did not (MMT Non-Hypo). No significant differences in oxyntomodulin, GIP or peptide YY secretion were seen between these two groups.

Conclusion: An early peak in GLP-1 and glucagon may together trigger an exaggerated insulinotropic response to eating and consequent hypoglycaemia in patients with PPH.

MeSH terms

  • Blood Glucose / analysis
  • Cohort Studies
  • Combined Modality Therapy / adverse effects
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / diet therapy
  • Diabetes Mellitus, Type 2 / surgery
  • Diet, Diabetic / adverse effects
  • Diet, Reducing / adverse effects
  • Female
  • Gastric Bypass / adverse effects*
  • Glucagon / blood*
  • Glucagon / metabolism
  • Glucagon-Like Peptide 1 / blood*
  • Glucagon-Like Peptide 1 / metabolism
  • Humans
  • Hypoglycemia / blood
  • Hypoglycemia / epidemiology
  • Hypoglycemia / etiology*
  • Hypoglycemia / prevention & control
  • Insulin / blood*
  • Insulin / metabolism
  • Insulin Secretion
  • London / epidemiology
  • Male
  • Middle Aged
  • Monitoring, Ambulatory
  • Obesity / complications
  • Obesity / diet therapy
  • Obesity / surgery
  • Postoperative Complications / blood
  • Postoperative Complications / epidemiology
  • Postoperative Complications / etiology*
  • Postoperative Complications / prevention & control
  • Postprandial Period
  • Prevalence
  • Risk Factors

Substances

  • Blood Glucose
  • Insulin
  • Glucagon-Like Peptide 1
  • Glucagon