LSD1 modulates the non-canonical integrin β3 signaling pathway in non-small cell lung carcinoma cells

Sci Rep. 2017 Aug 31;7(1):10292. doi: 10.1038/s41598-017-09554-x.

Abstract

The epigenetic writer lysine-specific demethylase 1 (LSD1) is aberrantly upregulated in many cancer types and its overexpression correlates with poor survival and tumor progression. In this study, we analysed LSD1 function in non-small cell lung cancer adenocarcinomas. Expression profiling of 182 cases of lung adenocarcinoma proved a significant correlation of LSD1 overexpression with lung adenocarcinoma progression and metastasis. KRAS-mutated lung cancer cell clones were stably silenced for LSD1 expression. RNA-seq and comprehensive pathway analysis revealed, that genes related to a recently described non-canonical integrin β3 pathway, were significantly downregulated by LSD1 silencing. Hence, invasion and self-renewal capabilities were strongly decreased. Notably, this novel defined LSD1/integrin β3 axis, was also detected in human lung adenocarcinoma specimens. Furthermore, the linkage of LSD1 to an altered expression pattern of lung-lineage specific transcription factors and genes, which are involved in alveolar epithelial differentiation, was demonstrated. Thus, our findings point to a LSD1-integrin β3 axis, conferring attributes of invasiveness and tumor progression to lung adenocarcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers
  • Biomarkers, Tumor
  • Carcinoma, Non-Small-Cell Lung / genetics
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cells, Cultured
  • Female
  • Gene Expression
  • Gene Knockdown Techniques
  • Histone Demethylases / genetics
  • Histone Demethylases / metabolism*
  • Humans
  • Immunohistochemistry
  • Integrin beta3 / metabolism*
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Mutation
  • NF-kappa B / metabolism
  • Neoplasm Grading
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Proto-Oncogene Proteins p21(ras) / metabolism
  • RNA, Messenger / genetics
  • Signal Transduction

Substances

  • Biomarkers
  • Biomarkers, Tumor
  • Integrin beta3
  • KRAS protein, human
  • NF-kappa B
  • RNA, Messenger
  • Histone Demethylases
  • KDM1A protein, human
  • Proto-Oncogene Proteins p21(ras)