High-Density Mapping of Brain Slices using a Large Multi-Functional High-Density CMOS Microelectrode Array System

We present a CMOS-based high-density microelectrode array (HD-MEA) system that enables high-density mapping of brain slices in-vitro with multiple readout modalities. The 4.48×2.43 mm² array consists of 59,760 micro-electrodes at 13.5 µm pitch (5487 electrodes/mm²). The overall system features 2048 action-potential, 32 local-field-potential and 32 current recording channels, 32 impedance-measurement and 28 neurotransmitter-detection channels and 16 voltage/current stimulation channels. The system enables real-time and label-free monitoring of position, size, morphology and electrical activity of brain slices.