LRP6 is identified as a potential prognostic marker for oral squamous cell carcinoma via MALDI-IMS

Yao Yuan; Xiaoyan Xie; Yuchen Jiang; Zihao Wei; Peiqi Wang; Fangman Chen; Xinyi Li; Chongkui Sun; Hang Zhao; Xin Zeng; Lu Jiang; Yu Zhou; Hongxia Dan; Mingye Feng; Rui Liu; Zhiyong Wang; Qianming Chen

doi:10.1038/cddis.2017.433

LRP6 is identified as a potential prognostic marker for oral squamous cell carcinoma via MALDI-IMS

Cell Death Dis. 2017 Sep 7;8(9):e3035. doi: 10.1038/cddis.2017.433.

Authors

Yao Yuan¹, Xiaoyan Xie², Yuchen Jiang¹, Zihao Wei¹, Peiqi Wang¹, Fangman Chen¹, Xinyi Li¹, Chongkui Sun¹, Hang Zhao¹, Xin Zeng¹, Lu Jiang¹, Yu Zhou¹, Hongxia Dan¹, Mingye Feng¹, Rui Liu¹, Zhiyong Wang¹, Qianming Chen¹

Affiliations

¹ State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
² Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, China.

Abstract

Oral squamous cell carcinoma (OSCC) is a leading cause of cancer-related deaths worldwide, with 500 000 new cases each year. However, the mechanisms underlying OSCC development are relatively unknown. In this study, matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)-based proteomic strategy was used to profile the differentially expressed peptides/proteins between OSCC tissues and their adjacent noncancerous tissues. Sixty-seven unique peptide peaks and five distinct proteins were identified with changed expression levels. Among them, LRP6 expression was found to be upregulated in OSCC tissues, and correlated with a cluster of clinicopathologic parameters, including smoking, drinking, tumor differentiation status, lymph node metastasis and survival time. Notably, knockdown of LRP6 inhibited the proliferation ability of OSCC cells. Furthermore, we demonstrated that the expression of LRP6 in OSCC cells is positively correlated with its downstream oncogene, FGF8. The present study suggests that LRP6 could be a potential biomarker for OSCC patients, and might further assist in the therapeutic decisions in OSCC treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Drinking / physiopathology
Biomarkers, Tumor / genetics*
Biomarkers, Tumor / metabolism
Carcinoma, Squamous Cell / diagnosis
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Cell Proliferation
Cigarette Smoking / physiopathology
Fibroblast Growth Factor 8 / genetics
Fibroblast Growth Factor 8 / metabolism
Gene Expression Regulation, Neoplastic*
Humans
Low Density Lipoprotein Receptor-Related Protein-6 / genetics*
Low Density Lipoprotein Receptor-Related Protein-6 / metabolism
Lymphatic Metastasis
Mouth Neoplasms / diagnosis
Mouth Neoplasms / genetics*
Mouth Neoplasms / mortality
Mouth Neoplasms / pathology
Neoplasm Proteins / genetics*
Neoplasm Proteins / metabolism
Neoplasm Staging
Prognosis
Proteome / genetics
Proteome / metabolism
Risk Factors
Signal Transduction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Survival Analysis

Substances

Biomarkers, Tumor
FGF8 protein, human
LRP6 protein, human
Low Density Lipoprotein Receptor-Related Protein-6
Neoplasm Proteins
Proteome
Fibroblast Growth Factor 8