Hydrophobic Collapse of the Intrinsically Disordered Transcription Factor Myc Associated Factor X

Biochemistry. 2017 Oct 10;56(40):5365-5372. doi: 10.1021/acs.biochem.7b00679. Epub 2017 Sep 21.

Abstract

The conformational space of the proto-oncogenic transcription factor Myc associated factor X (MAX) comprises a dynamic equilibrium between a stably folded coiled-coil homodimer and an intrinsically disordered ensemble of states. We show by means of nuclear magnetic resonance spectroscopy that the intrinsically disordered ensemble samples structures that are even as compact as the folded dimer. These extremely dense, hydrophobically collapsed globules might be of importance for interconversion between different conformations of intrinsically disordered proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic-Leucine Zipper Transcription Factors / chemistry*
  • Hydrophobic and Hydrophilic Interactions*
  • Intrinsically Disordered Proteins / chemistry*
  • Models, Molecular
  • Protein Conformation
  • Protein Stability

Substances

  • Basic-Leucine Zipper Transcription Factors
  • Intrinsically Disordered Proteins
  • Myc associated factor X