The novel dopamine D3 receptor antagonists/partial agonists CAB2-015 and BAK4-54 inhibit oxycodone-taking and oxycodone-seeking behavior in rats

Neuropharmacology. 2017 Nov:126:190-199. doi: 10.1016/j.neuropharm.2017.09.007. Epub 2017 Sep 6.

Abstract

The use of prescription opioid analgesics, particularly oxycodone, has dramatically increased, and parallels escalated opioid abuse and drug-related deaths worldwide. Understanding the molecular mechanisms underlying the development of opioid dependence and expanding treatment options to counter prescription opioid abuse has become a critical public health matter. In the present study, we first evaluated the reinforcing effects of oxycodone in a rat model of self-administration and then explored the potential utility of two novel high affinity dopamine D3 receptor (D3R) antagonists/partial agonists, CAB2-015 and BAK4-54, for treatment of prescription opioid abuse and dependence. We found that rats acquired oxycodone self-administration rapidly within a range of unit doses that was similar to that for heroin, confirming that oxycodone has significant abuse potential. Strikingly, pretreatment with either CAB2-015 or BAK4-54 (0.4-10 mg/kg, i.p.) dose-dependently decreased oxycodone self-administration, and shifted the oxycodone dose-response curve downward. Repeated pretreatment with CAB2-015 or BAK4-54 (0.4-4 mg/kg) facilitated extinction and inhibited oxycodone-induced reinstatement of drug-seeking behavior. In addition, pretreatment with CAB2-015 or BAK4-54 (4-10 mg/kg) also dose-dependently decreased oxycodone-enhanced locomotor activity, but only CAB2-015 decreased oral sucrose self-administration. These data suggest that D3R antagonists may be suitable alternatives or adjunctive to opioid-based medications currently used clinically in treating opioid addiction and that the D3R-selective ligands (CAB2-015 or BAK4-54) provide new lead molecules for development.

Keywords: BAK4-54; CAB2-015; Dopamine D3 receptor antagonist; Oxycodone; Prescription opioid; Self-administration.

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Drug Partial Agonism
  • Drug-Seeking Behavior / drug effects*
  • Extinction, Psychological / drug effects
  • Heroin / administration & dosage
  • Indoles / pharmacology*
  • Male
  • Naphthalenes / pharmacology*
  • Narcotics / administration & dosage*
  • Opioid-Related Disorders / prevention & control*
  • Oxycodone / administration & dosage*
  • Piperazines / pharmacology*
  • Quinolines / pharmacology*
  • Rats, Long-Evans
  • Receptors, Dopamine D3 / agonists*
  • Receptors, Dopamine D3 / antagonists & inhibitors*
  • Self Administration

Substances

  • BAK4-54
  • CAB2-015
  • Indoles
  • Naphthalenes
  • Narcotics
  • Piperazines
  • Quinolines
  • Receptors, Dopamine D3
  • Heroin
  • Oxycodone