Natural products and morphogenic activity of γ-Proteobacteria associated with the marine hydroid polyp Hydractinia echinata

Bioorg Med Chem. 2017 Nov 15;25(22):6088-6097. doi: 10.1016/j.bmc.2017.06.053. Epub 2017 Jul 1.

Abstract

Illumina 16S rRNA gene sequencing was used to profile the associated bacterial community of the marine hydroid Hydractinia echinata, a long-standing model system in developmental biology. 56 associated bacteria were isolated and evaluated for their antimicrobial activity. Three strains were selected for further in-depth chemical analysis leading to the identification of 17 natural products. Several γ-Proteobacteria were found to induce settlement of the motile larvae, but only six isolates induced the metamorphosis to the primary polyp stage within 24h. Our study paves the way to better understand how bacterial partners contribute to protection, homeostasis and propagation of the hydroid polyp.

Keywords: Hydractinia echinata; Indoles; Metamorphosis; Secondary metabolites; Signaling molecules.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Bacteria / classification
  • Bacteria / genetics*
  • Biological Products / chemistry*
  • Biological Products / isolation & purification
  • Biological Products / pharmacology
  • Chromatography, High Pressure Liquid
  • Gammaproteobacteria / classification
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / metabolism*
  • High-Throughput Nucleotide Sequencing
  • Hydrozoa / growth & development
  • Hydrozoa / microbiology*
  • Larva / microbiology
  • Mass Spectrometry
  • Metamorphosis, Biological / drug effects
  • Phylogeny
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Ribosomal, 16S / classification
  • RNA, Ribosomal, 16S / metabolism
  • Sequence Analysis, DNA

Substances

  • Biological Products
  • RNA, Ribosomal, 16S