Transient transfection of Cryptosporidium baileyi

A-K Tetens; S Hanig; M Kurth; G Greif; R Entzeroth

doi:10.1016/j.parint.2017.09.005

Transient transfection of Cryptosporidium baileyi

Parasitol Int. 2017 Dec;66(6):813-816. doi: 10.1016/j.parint.2017.09.005. Epub 2017 Sep 12.

Authors

A-K Tetens¹, S Hanig², M Kurth², G Greif³, R Entzeroth²

Affiliations

¹ Institute of Systematic Zoology and Parasitology, Faculty of Science, Technische Universität, Dresden, Dresden, Germany. Electronic address: [email protected].
² Institute of Systematic Zoology and Parasitology, Faculty of Science, Technische Universität, Dresden, Dresden, Germany.
³ Bayer Animal Health GmbH, 51368 Leverkusen, Germany.

PMID: 28916308
DOI: 10.1016/j.parint.2017.09.005

Abstract

Here we demonstrate the transient transfection of C. baileyi. We describe the transfection of this apicomplexan parasite and the cultivation in ovo. The functionality of heterologous sequences in C. baileyi was demonstrated by the expression and detection of the mCherry protein in ovo. The mCherry protein was expressed in parasitic stages up to the oocyst stage under the control of the heterologous promoter region of the C. parvum actin gene.

Keywords: Apicomplexa; Cryptosporidium; Heterologous; In ovo; Transfection.

MeSH terms

Cryptosporidium / genetics
Luminescent Proteins / analysis
Oocysts / genetics
Red Fluorescent Protein
Transfection / methods*

Substances

Luminescent Proteins