A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs

Diagn Microbiol Infect Dis. 2017 Nov;89(3):182-184. doi: 10.1016/j.diagmicrobio.2017.08.007. Epub 2017 Aug 17.

Abstract

A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.

Keywords: Chlamydia trachomatis; Diagnosis; Quantitative PCR; Sample prep; Sexually transmitted disease.

MeSH terms

  • Cervix Uteri / microbiology
  • Chlamydia Infections / diagnosis*
  • Chlamydia Infections / microbiology*
  • Chlamydia trachomatis / genetics*
  • Chlamydia trachomatis / isolation & purification
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / isolation & purification
  • Female
  • Humans
  • Pilot Projects
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity

Substances

  • DNA, Bacterial