Abstract
We characterized the tension response of clathrin-mediated endocytosis by using various cell manipulation methodologies. Elevated tension in a cell hinders clathrin-mediated endocytosis through inhibition of de novo coat initiation, elongation of clathrin coat lifetimes and reduction of high-magnitude growth rates. Actin machinery supplies an inward pulling force necessary for internalization of clathrin coats under high tension. These findings suggest that the physical cues cells receive from their microenvironment are major determinants of clathrin-mediated endocytic activity.
Keywords:
Actin dynamics; Clathrin-mediated endocytosis; Hypotonic shock; Membrane tension; Microaspiration.
© 2017. Published by The Company of Biologists Ltd.
MeSH terms
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Actins / genetics
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Actins / metabolism*
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Animals
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Biomechanical Phenomena
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Cell Line
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Cell Line, Tumor
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Cell Size
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Chlorocebus aethiops
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Clathrin / genetics
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Clathrin / metabolism*
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Clathrin-Coated Vesicles / metabolism*
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Clathrin-Coated Vesicles / ultrastructure
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Coated Pits, Cell-Membrane / metabolism
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Coated Pits, Cell-Membrane / ultrastructure
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Endocytosis / physiology*
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Epithelial Cells / metabolism*
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Epithelial Cells / ultrastructure
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Fatty Acid-Binding Proteins / genetics
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Fatty Acid-Binding Proteins / metabolism
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Female
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Gene Expression
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Kidney / cytology
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Kidney / metabolism
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Mechanotransduction, Cellular*
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Osmotic Pressure
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Stress, Mechanical
Substances
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Actins
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Clathrin
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Fatty Acid-Binding Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins