Generation of integration-free induced pluripotent stem cells (GZHMUi001-A) by reprogramming peripheral blood mononuclear cells from a 47, XXX syndrome patient

Stem Cell Res. 2017 Aug:23:57-60. doi: 10.1016/j.scr.2017.06.002. Epub 2017 Jun 9.

Abstract

47, XXX syndrome is one of several sex-chromosomal aneuploidies, and it has an incidence of approximately 1/1000 in newborn females. Because of heterogeneity in X-inactivation, these patients may exhibit a variety of clinical symptoms. Here, we report the generation of an integration-free human induced pluripotent stem cell line (GZHMUi001-A) by using Sendai virus to reprogram peripheral blood mononuclear cells from a 47, XXX syndrome patient with premature ovarian failure. This 47, XXX iPS cell line has characteristics of pluripotent stem cells and is a useful tool for the investigation of this X chromosome aneuploid disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cellular Reprogramming*
  • Chromosomes, Human, X
  • Female
  • Humans
  • Induced Pluripotent Stem Cells / pathology*
  • Leukocytes, Mononuclear / pathology*
  • Sex Chromosome Aberrations
  • Sex Chromosome Disorders of Sex Development / pathology*
  • Trisomy / pathology*
  • Young Adult

Supplementary concepts

  • Triple X syndrome