Luciferases with Tunable Emission Wavelengths

Julien Hiblot; Qiuliyang Yu; Marina D B Sabbadini; Luc Reymond; Lin Xue; Alberto Schena; Olivier Sallin; Nicholas Hill; Rudolf Griss; Kai Johnsson

doi:10.1002/anie.201708277

Luciferases with Tunable Emission Wavelengths

Angew Chem Int Ed Engl. 2017 Nov 13;56(46):14556-14560. doi: 10.1002/anie.201708277. Epub 2017 Oct 9.

Authors

Julien Hiblot^{1

2}, Qiuliyang Yu^{1

2}, Marina D B Sabbadini¹, Luc Reymond¹, Lin Xue^{1

2}, Alberto Schena¹, Olivier Sallin¹, Nicholas Hill¹, Rudolf Griss¹, Kai Johnsson^{1

2}

Affiliations

¹ Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
² Max-Planck-Institute for Medical Research, Department of Chemical Biology, 69120, Heidelberg, Germany.

PMID: 28941028
DOI: 10.1002/anie.201708277

Abstract

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

Keywords: bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques
Fluorescent Dyes / chemistry
Genes, Reporter
HeLa Cells
Humans
Luciferases / chemistry*
Luminescent Measurements / methods

Substances

Fluorescent Dyes
Luciferases