Crystal structure of the WD40 domain of human PRPF19

Yuzhe Zhang; Yue Li; Xiao Liang; Zhongliang Zhu; Hongbin Sun; Hao He; Jinrong Min; Shanhui Liao; Yanli Liu

doi:10.1016/j.bbrc.2017.09.145

Crystal structure of the WD40 domain of human PRPF19

Biochem Biophys Res Commun. 2017 Nov 25;493(3):1250-1253. doi: 10.1016/j.bbrc.2017.09.145. Epub 2017 Sep 28.

Authors

Yuzhe Zhang¹, Yue Li², Xiao Liang³, Zhongliang Zhu², Hongbin Sun⁴, Hao He⁵, Jinrong Min⁶, Shanhui Liao⁷, Yanli Liu⁸

Affiliations

¹ Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China; Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada; Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali 671000, PR China.
² School of Life Sciences, University of Science and Technology of China, Hefei 230027, PR China.
³ Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
⁴ High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Academy of Sciences of China, Hefei 230031, PR China.
⁵ Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada.
⁶ Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China; Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada.
⁷ School of Life Sciences, University of Science and Technology of China, Hefei 230027, PR China. Electronic address: [email protected].
⁸ Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China; Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada. Electronic address: [email protected].

PMID: 28962858
DOI: 10.1016/j.bbrc.2017.09.145

Abstract

Human Pre-mRNA Processing factor 19 (hPRPF19) is an important component in human spliceosome machinery. hPRPF19 contains a WD40 repeats domain at its C-terminus, which is also conserved in yeast. Here we determined the crystal structure of the C-terminal WD40 repeat domain of hPRPF19 by X-ray crystallography. Our structural analysis revealed some significantly different structure features between the human and yeast Prp19 WD40 repeat domain. However, there are also conserved clusters of residues at the bottom surface of the fourth and the fifth WD40 repeats, which provides the important implication for the conserved Prp19 proteins in both human and yeast.

Keywords: Crystal structure; Human Pre-mRNA-processing factor 19 (hPRPF19); Spliceosome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallography, X-Ray
DNA Repair Enzymes / chemistry*
DNA Repair Enzymes / genetics
DNA Repair Enzymes / metabolism
Humans
Models, Molecular
Nuclear Proteins / chemistry*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Protein Conformation
Protein Domains
RNA Splicing Factors / chemistry*
RNA Splicing Factors / genetics
RNA Splicing Factors / metabolism
Saccharomyces cerevisiae Proteins / chemistry
WD40 Repeats

Substances

Nuclear Proteins
PRP19 protein, S cerevisiae
RNA Splicing Factors
Saccharomyces cerevisiae Proteins
DNA Repair Enzymes
PRPF19 protein, human

Abstract

Publication types

MeSH terms

Substances

Grants and funding