Comparison of three molecular assays for detection of enteric viruses in stool samples

J Virol Methods. 2017 Dec:250:55-58. doi: 10.1016/j.jviromet.2017.09.026. Epub 2017 Sep 29.

Abstract

Three molecular assays (FTD® Viral GE from Fast-track diagnostics, RIDA®GENE VSP1 from R-Biopharm, and Xpert Norovirus from Cepheid) were compared for virus detection in acute diarrhea samples. RIDA®GENE and FTD® Viral GE showed perfect/almost perfect agreement for Rotavirus, Sapovirus and Norovirus, substantial agreement for Adenovirus, and moderate agreement for Astrovirus.

Keywords: Adenovirus; Astrovirus; Molecular assay; Norovirus; Rotavirus; Sapovirus.

Publication types

  • Comparative Study

MeSH terms

  • Adenoviridae / genetics
  • Adenoviridae / isolation & purification
  • Adult
  • Aged
  • Aged, 80 and over
  • Astroviridae / genetics
  • Astroviridae / isolation & purification
  • Body Fluids / virology
  • Child
  • Child, Preschool
  • Diarrhea / virology
  • Enterovirus Infections / diagnosis*
  • Enterovirus Infections / virology
  • Feces / virology*
  • Female
  • Gastroenteritis / virology
  • Humans
  • Infant
  • Male
  • Mamastrovirus / genetics
  • Mamastrovirus / isolation & purification
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • RNA, Viral
  • Rotavirus / genetics
  • Rotavirus / isolation & purification*
  • Sapovirus / genetics
  • Sapovirus / isolation & purification*
  • Young Adult

Substances

  • RNA, Viral