Characterization of a monoclonal antibody to guinea pig T cells that inhibits rosette formation of the cells with rabbit erythrocytes: similarity of the antigen to E-receptor on human T cells

Microbiol Immunol. 1988;32(2):187-98. doi: 10.1111/j.1348-0421.1988.tb01377.x.

Abstract

A monoclonal antibody, GPT-1, was prepared by fusion of the splenic cells of mice immunized with guinea pig thymocytes with a mouse myeloma cell line. GPT-1 completely inhibited spontaneous rosette formation of T cells with papain-treated rabbit erythrocytes. GPT-1 reacted with 90% of thymocytes, 70% of peripheral blood lymphocytes, and 45% of splenic lymphocytes, but not with B cells. These results indicate that GPT-1 has pan-T reactivity. The antibody specifically bound to a single polypeptide chain with a molecular size of 50-65 kD. The surface density of the antigen was higher on thymocytes than on peripheral T cells, suggesting that the antigen is a certain differentiation antigen on T cells. Phytohemagglutinin-activated T cells expressed more antigen molecules than resting T cells. In addition, GPT-1 suppressed the proliferation of T cells induced by the mitogen, indicating that GPT-1 recognizes a T cell-specific surface antigen which is associated with T cell activation. Based on these results, it was concluded that GPT-1 reacts with a guinea pig T cell surface antigen which is similar to the E-receptor protein on human T cells (CD2 molecule).

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Differentiation / immunology*
  • Antigens, Surface / immunology
  • CD2 Antigens
  • Erythrocytes / immunology
  • Guinea Pigs
  • Humans
  • In Vitro Techniques
  • Lymphocyte Activation
  • Male
  • Phytohemagglutinins / pharmacology
  • Rabbits
  • Receptors, Immunologic / immunology*
  • Rosette Formation
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation
  • Antigens, Surface
  • CD2 Antigens
  • Phytohemagglutinins
  • Receptors, Immunologic