Mechano growth factor E peptide inhibits invasion of melanoma cells and up-regulates CHOP expression via endoplasmic reticulum stress

Biotechnol Lett. 2018 Jan;40(1):205-213. doi: 10.1007/s10529-017-2448-5. Epub 2017 Oct 6.

Abstract

Objective: To evaluate the effects of mechano growth factor E peptide (MGF) on the invasive properties of melanoma cells.

Results: Melanoma cells (GLL19) were treated with 10, 20 and 30 ng MGF/ml for 24 h. Their invasive properties were investigated by transwell assay. Cytoskeleton reorganization was assessed via staining with phalloidin-FITC; lysyl oxidase (LOX) family gene expression was tested by qRT-PCR, and western blotting was used to detect expression of the matrix metalloproteinases (MMPs) and endoplasmic reticulum (ER) stress. MGF decreased the invasive capabilities of melanoma cells and induced changes in cytoskeleton distribution. MGF also down-regulated the expression of MMPs and up-regulated the expression of the cell apoptosis-related protein CHOP by inducing ER stress.

Conclusions: MGF can decrease the invasive properties of melanoma cells and induce ER stress, promoting cell apoptosis. Thus, MGF represents a novel strategy for the potential treatment of patients presenting with cutaneous melanoma.

Keywords: C/EBP homologous protein (CHOP); Endoplasmic reticulum stress; Invasion; Mechano growth factor; Melanoma.

MeSH terms

  • Biological Factors / metabolism*
  • Cell Line, Tumor
  • Cell Movement*
  • Cell Proliferation
  • Cytoskeleton / metabolism
  • Endoplasmic Reticulum Stress / drug effects*
  • Humans
  • Insulin-Like Growth Factor I / metabolism*
  • Melanocytes / drug effects*
  • Melanoma
  • Transcription Factor CHOP / biosynthesis*

Substances

  • Biological Factors
  • DDIT3 protein, human
  • mechano-growth factor E, human
  • Transcription Factor CHOP
  • Insulin-Like Growth Factor I