Evaluation of Pyridaben Residues on Fruit Surfaces and Their Stability by a Novel On-Line Dual-Frequency Ultrasonic Device and Chemiluminescence Detection

In this paper, we first report the development of a highly sensitive and economical method for accurate analysis of pyridaben residues on fruits based on dual-frequency ultrasonic treatment (DFUT) and flow injection chemiluminescence (CL) detection. The DFUT device is made by integrating an ultrasonic bath with an ultrasonic probe. Two quartz glass coils (QGC) with different structures have been designed and applied to evaluate the function of DFUT in the detection process. Recorded data showed that DFUT is an effective method for improving the pyridaben CL signal. The signal of pyridaben in response to DFUT is 2.0-3.3 times stronger than the response to only the ultrasonic probe at 20 kHz or the ultrasonic bath at 40 kHz. In addition, the response obtained from the concentric circle QGC is 2.1 times stronger than the response to the spiral tube QGC. Under the optimized condition, the proposed method has advantages, such as a wide linear range (0.8-100.0 μg L^-1), a high sensitivity (limit of detection of 0.085 μg L^-1), and good stability (RSDs ≤ 4.7% in the linear range) for pyridaben determination. We apply this method to monitor the residue pyridaben on some fruits. The data show that the maximum amounts of the residue on fruit surfaces after soaking in water (50 mg L^-1, 5 min) are 0.583 mg kg^-1 (apple), 0.794 mg kg^-1 (orange), and 0.351 mg kg^-1 (pear). However, the concentration of pyridaben in the presence of sunlight decreases rapidly, showing its poor light stability.