Integrin β-3 is required for the attachment, retention and therapeutic benefits of human cardiospheres in myocardial infarction

J Cell Mol Med. 2018 Jan;22(1):382-389. doi: 10.1111/jcmm.13325. Epub 2017 Oct 11.

Abstract

Cardiovascular diseases remain the leading causes of death worldwide. Stem cell therapy offers a promising option to regenerate injured myocardium. Among the various types of stem cells, cardiosphere cells represent a mixture of intrinsic heart stem cells and supporting cells. The safety and efficacy of cardiosphere cells have been demonstrated in recent clinical trials. Cell-matrix interaction plays an important role in mediating the engraftment of injected stem cells. Here, we studied the role of integrin β-3 in cardiosphere-mediated cell therapy in a mouse model of myocardial infarction. Our results indicated that inhibiting integrin β-3 reduced attachment, retention and therapeutic benefits of human cardiospheres in mice with acute myocardial infarction. This suggests integrin β-3 plays an important role in cardiosphere-mediated heart regeneration.

Keywords: cardiac stem cells; cardiospheres; integrin; myocardial infarction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / pharmacology
  • Cell Adhesion / drug effects
  • Cell Survival / drug effects
  • Humans
  • Integrin beta3 / metabolism*
  • Male
  • Mice, SCID
  • Myocardial Infarction / pathology
  • Myocardial Infarction / therapy*
  • Myocytes, Cardiac / drug effects
  • Myocytes, Cardiac / transplantation*
  • Phenotype
  • Spheroids, Cellular / cytology
  • Spheroids, Cellular / transplantation*

Substances

  • Antibodies
  • ITGB3 protein, human
  • Integrin beta3