Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system

Kyung-Ok Uhm; Gue Youn Go; So-Jung Kim; Eun Hee Jo; Hye Young Choi; Young Sam Im; Hye-Yeong Ha; Jung-Hyun Kim; Soo Kyung Koo

doi:10.1016/j.scr.2017.02.009

Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system

Stem Cell Res. 2017 Oct:24:148-150. doi: 10.1016/j.scr.2017.02.009. Epub 2017 Feb 24.

Authors

Kyung-Ok Uhm¹, Gue Youn Go¹, So-Jung Kim¹, Eun Hee Jo¹, Hye Young Choi¹, Young Sam Im¹, Hye-Yeong Ha¹, Jung-Hyun Kim¹, Soo Kyung Koo²

Affiliations

¹ Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
² Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea. Electronic address: [email protected].

PMID: 29034882
DOI: 10.1016/j.scr.2017.02.009

Abstract

We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model. These two cell lines are registered and available from the National Stem Cell Bank, Korea National Institute of Health.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Fibroblasts / metabolism*
Humans
Induced Pluripotent Stem Cells / metabolism*
Male
RNA / metabolism*

Substances

RNA