Abstract
We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM. In addition, upregulation of IL-8 and GM-CSF and downregulation of RANTES expression were shown in response to SFXFM. Moreover, two-lineage differentiation properties (osteocyte and adipocyte) of hTMSCs were enhanced under SFXFM. Finally, the genetic stability of SFXFM-cultured hTMSCs was demonstrated by normal karyotype results. SFXFM enables good expansion, multipotentiality, and normal genotype maintenance of MSCs. Moreover, this approach serves as a substitute to conventional media for the cultivation of capable MSCs for upcoming medical applications.
MeSH terms
-
Cell Differentiation
-
Cell Proliferation
-
Cell Separation
-
Cells, Cultured
-
Chemokine CCL5 / metabolism
-
Culture Media, Serum-Free
-
Flow Cytometry
-
Genomic Instability
-
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
-
Humans
-
Interleukin-8 / metabolism
-
Mesenchymal Stem Cells / cytology*
-
Mesenchymal Stem Cells / metabolism
-
Turbinates / cytology*
Substances
-
Chemokine CCL5
-
Culture Media, Serum-Free
-
Interleukin-8
-
Granulocyte-Macrophage Colony-Stimulating Factor
Grants and funding
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A02936924), by the National Research Foundation of Korea grant funded by the Ministry of Science, ICT and Future Planning (no. 2014R1A2A2A01004325), and the Institute of Clinical Medicine Research of Bucheon St. Mary's Hospital, Research Fund, 2016 and 2017. This research was also supported by a grant of the E.N.T. Fund of the Catholic University of Korea made in the program year of 2016 and 2017. Additionally, This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C3228, HI15C1199, and HI16C0133).